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KMID : 1134820180470111069
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Journal of the Korean Society of Food Science and Nutrition
2018 Volume.47 No. 11 p.1069 ~ p.1075
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Immunomodulatory Activity of Platycodon grandiflorum, Codonopsis lanceolata, and Adenophora triphylla Extracts in Macrophage Cells
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Kim Eiun-Hye
Kwak Ji-Young
Chung Mi-Ja
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Abstract
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This study investigated the immunomodulatory and anti-inflammatory activities of water extract of Platycodon grandiflorum, Codonopsis lanceolata, and Adenophora triphylla (PG, CL, AT) in a murine RAW264.7 macrophage model. As immuno response biomarkers, the cell proliferation, nitric oxide (NO) production, tumor necrosis factor (TNF)-α production and degree of mitogen-activated protein kinase (MAPKs) phosphorylation in RAW264.7 macrophage cells were evaluated. PG and CL increased macrophage proliferation and production of NO and TNF-α, with greater immunostimulatory activities being observed in response to PG than CL in RAW264.7 cells. The increase of NO and TNF-α in PG or CL-treated cells was associated with phosphorylation of MAPKs, including extracellular signal-regulated kinase (ERK), p38 and c-Jun N-terminal kinase (JNK) in RAW264.7 cells. The phosphorylation of ERK, p38 and JNK (p-ERK, p-p38, p-JNK) of RAW264.7 cells treated with PG and CL, respectively, also increased significantly. The NO and TNF-α production and phosphorylation of ERK and p38 were unchanged in AT-treated RAW264.7 macrophages. Furthermore, we examined the effects of PG, CL and AT on NO and TNF-α production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. PG, CL and AT inhibited LPS-induced NO and TNF-α production. These findings suggest that PG and CL can induce macrophage activation through MAPK signaling as immunomodulators and that they exert anti-inflammatory effects through inhibition of NO and TNF-α production.
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KEYWORD
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anti-inflammatory activity, Codonopsis lanceolata, immunomodulatory activity, macrophages, Platycodon grandiflorum
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